Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 μg per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4°C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kDa, 42 kDa