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  • 產(chǎn)品名稱：Mouseanti-HumanIgMAntibody

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  • 產(chǎn)品廠商：NSJBio
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簡單介紹：
Mouseanti-HumanIgMAntibody
詳情介紹：
Immunogen Recombinant human IgM was used as the immunogen for this anti-IgM antibody.
Clone IM260
Isotype IgG1, kappa
Purification Protein G
Background IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen s (due to the large size of most antigens, not all s can be filled simultaneously. IgM antibodies account for approximately 5?% -10?% of all the antibody in the body.

This mAb is specific for the human IgM heavy chain. It does not cross-react with other immunoglobulin heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. IgM antibody is useful in the identification of certain cancer types. Some tumors express a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Research Area Secondary Antibodies
Application Notes Due to differences in protocols and secondaries, the anti-IgM antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling sections in 10?mM citrate buffer,?pH 6.0, for 10-20?min followed by cooling at RT for 20?minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30?min.\. FACS: 0.5-1?μg/10e6 cells,IF: 0.5-1?μg/mL,WB: 0.5-1?μg/mL,IHC (FFPE): 0.5-1?μg/mL for 30?minutes at RT (1),Prediluted format : incubate for 30?min at RT (2)
Restrictions For Research Use only
Buffer 1?mg/mL in 1X PBS, BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment Store the anti-IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).