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產(chǎn)品名稱：Goatanti-RabbitIgG(Heavy&LightChain)Antibody(IRDye800CW)
產(chǎn)品型號：
產(chǎn)品廠商：LI-COR
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簡單介紹：

Goatanti-RabbitIgG(Heavy&LightChain)Antibody(IRDye800CW)

詳情介紹：

Product details
Product details
Brand	In-Cell Western?,CellTag?
Immunogen	Rabbit IgG
Isotype	IgG
Fragment	Whole molecule
Specificity	Rabbit IgG Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA.
Cross-Reactivity (Details)	This antibody was tested by Dot Blot and/or solid-phase adsorbed for minimal cross-reactivity with human, mouse, rat, sheep, and chicken serum proteins, but may cross-react with immunoglobulins from other species.
Characteristics	The conjugate has been specifically tested and qualified for Western blotting applications.
Purification	immunoaffinity chromatography

Target details
Target details
Research Area	Immunology, Secondary Antibodies

Application Details
Application Details
Application Notes	Western blot: 1:5,000 - 1:25,000
Restrictions	For Research Use only

Handling
Handling
Format	Lyophilized
Reconstitution	Reconstitute with sterile, distilled water
Concentration	1.0 mg/mL
Buffer	PBS,?pH 7.4
Preservative	Sodium azide
Precaution of Use	This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice	Protect from light.
Storage	4 °C

Images
Images
Supplier Images	Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with m... Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with se... The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-M... ERK (green) and pERK (red) multiplex detection using infrared dye-labeled secondary a... p-38 (red) and Tubulin (green) multiplex detection using infrared dye-labeled seconda... Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot wa... Images for product: Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (IRDye800CW) Nuclear enriched lysate probed for alpha-tubulin and ELF5. The blot was probed with mouse anti-alpha-tubulin and rabbit anti-ELF5 followed by detection with IRDye? 680RD Goat anti-Mouse IgG and IRDye 800CW Goat anti-Rabbit IgG. Dose response of EGFR phoshphorylation in A431 cells. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Lysates were resolved by SDS-PAGE and transferred to Odyssey? Nitrocellulose Membrane. The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye? 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-Mouse IgG. The blot was probed with IRDye 800CW Goat anti-Rabbit IgG and IRDye 680RD Goat anti-Mouse IgG ERK (green) and pERK (red) multiplex detection using infrared dye-labeled secondary antibodies. p-38 (red) and Tubulin (green) multiplex detection using infrared dye-labeled secondary antibodies Effect of siRNA and cytokine treatment on phosphorylation of AKT and ERK. The blot was probed with rabbit anti-ERK, mouse anti-pERK, mouse anti-AKT, and rabbit anti-pAKT primary antibodies. IRDye? 800CW goat anti-rabbit IgG and IRDye 680LT goat anti-mouse IgG were used for detection.

References
References
Product cited in:	Malinverni, Unterreiner, Staal, Demeyer, Galaup, Luyten, Beyaert, Bornancin: "Cleavage by MALT1 induces cytosolic release of A20." in: Biochemical and biophysical research communications, Vol. 400, Issue 4, pp. 543-7, 2010 (PubMed).

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