產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：Mu-F-HPh-FMK(CalpainInhibitor2)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：KamiyaBiomedical
  • 產(chǎn)品文檔：
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Mu-F-HPh-FMK(CalpainInhibitor2)
詳情介紹：
Sequence Mu-Phe-HPh-CH2F, Mu-F-hF-FMK
Specificity Specific inhibition of calpains I and II. Also inhibits cathepsin L.
Characteristics Peptide-fluoromethyl ketone inhibitor 2 of calpains I and II and cathepsin L. The FMK (fluoromethyl ketone, CH2F) inhibitor has several advantages over other types of derivatives: penetrates cell membranes, is non-toxic to cells, irreversible inhibition.
Formula C25H30O4N3F
Solubility DMSO
Molecular Weight 455 Da
Application Notes It can be used for the inhibition of calpain activity in vitro. In animal studies, the inhibitor can be used in vivo.
Comment

Dissolve the inhibitor at 20 mM
in high purity (>99.9%) DMSO before use.

For use on intact cells:
1. Prepare desired concentrated stock solutions as follows:
5 mg Mu-F-HPh-FMK
in 55μl DMSO = 20 mM
in 1,10μl DMSO = 10 mM
in 2,20μl DMSO = 5 mM, etc.

2. Adding 2 μL of a 10 mM stock solution to 1 mL of culture medium gives a final Mu-F-HPh- FMK concentration of 20 μM.
Levels of DMSO above 0.2% may cause some cellular toxicity in culture medium, thus masking the effect of the protease inhibitor. For extended use in vitro or in vivo in animal studies: For experiments extending 12 to 48 hours, fresh inhibitor may have to be added (injected) due to inactivation of the inhibitor by endogenous cysteine proteases.
Restrictions For Research Use only
Format Solid
Storage -20 °C