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Purpose
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The reagent is supplied at a concentration of ~5mM. Prepare a 500-fold dilution in phosphate buffer (0.1 M phosphate, pH 7.4) at time of use.
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Specificity
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DAR-4M AM is a reagent used for the fluorescent detection of nitric oxide (NO). It is cell permeable and can be used to detect NO directly in cell specimens prepared from various sources, such as the brain. (Ex: ~560 nm, EM: ~ 575 nm).
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Characteristics
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To avoid moisture absorption allow vial to reach room temperature before opening. The detection limit is about 7 nM. Assay (wavelength): Excitation max. 560 nm, emission max. 575 nm.
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Purity
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98 % by HPLC
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Chemical Name
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Diaminorho damine-4M acetoxymet hyl ester
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Formula
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C28H31IN4O5
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Permeability
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Cell-permeable
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Solubility
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Soluble in DMSO
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