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簡單介紹：
HumanGalectin-1ELISAPairSet
詳情介紹：
Purpose The Human Galectin-1 ELISA Pair Set is for the quantitative determination of Human Galectin-1.
This ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay).
Detection Method Colorimetric
Sensitivity 312.5 pg/mL
Characteristics Capture Antibody: 0.5?mg/mL of rabbit anti-Human Galectin-1 monoclonal antibody (in PBS,?pH 7.4). Dilute to a working concentration of 2?μg/mL in PBS before coating.

Detection Antibody: 0.2?mg/mL of rabbit anti-Human Galectin-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50?% HRP-Protector,?pH 7.4, store at 4?°C). Dilute to working concentration of 0.5?μg/mL in detection antibody dilution buffer before use.

Standard: Each vial contains 870?ng of recombinant Human Galectin-1. Reconstitute with 1?mL detection antibody dilution buffer. After reconstitution, store at -20?°C to -80?°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20000 pg/mL is recommended.

Sensitivity: The minimum detectable dose of Human Galectin-1 was determined to be approximately 312.5 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Components
  • Capture Ab
  • Detection Ab
  • Standard
Alternative Name LGALS1
Application Notes Optimal working dilution should be determined by the investigator.
Protocol This ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Galectin-1 coated on a 96-well plate. Standards and samples are added to the wells, and any Human Galectin-1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human Galectin-1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Galectin-1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Restrictions For Research Use only
Format Liquid
Storage 4 °C,-20 °C,-80 °C
Storage Comment Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Supplier Images
ELISA image for Human Galectin-1 ELISA Pair Set (ABIN5499359) Human Galectin-1/LGALS1 ELISA Pair Set Standard Curve 7096