產(chǎn)品詳情
簡(jiǎn)單介紹:
Rabbitanti-Cat(Feline)IgG(FcRegion)Antibody(HRP)
詳情介紹:
Immunogen | Cat IgG F(c) fragment |
Purification |
Immunoaffinity chromatography. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Background | Synonyms: Cat IgG, Cat Immunoglobulin G |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
ELISA: 1/10000 - 1/50000. Western Blot: 1/1000 - 1/10000. Immunohistochemistry: 1/500 - 1/2500. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
Reconstitution | Restore with 1.0?mL of deionized water (or equivalent) |
Concentration | 2.0 mg/mL |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2 with 10?mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01?% (w/v) Gentamicin Sulfate as preservative |
Preservative | Gentamicin sulfate |
Precaution of Use | This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. |
Handling Advice |
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. |
Storage | -20 °C |
Storage Comment | Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-0 °C for one month or (in aliquots) at -20 °C for longer. |
Background publications |
Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).
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